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The HEK293 parental cell line originates from human embryonic kidney tissue and has been transformed with adenovirus type 5 (Ad5). This polyclonal pool carries heterogeneous gene disruptions introduced by CRISPR/Cas9-mediated genome editing, providing a robust model for investigating HASPIN-dependent cellular processes without the clonal bias inherent in single-cell-derived knockouts. The HASPIN Knockout HEK293 Polyclonal Cells represent a CRISPR/Cas9-edited polyclonal knockout cell population derived from the human embryonic kidney cell line HEK293, engineered for loss-of-function studies of the serine/threonine kinase HASPIN. Applications include immunofluorescence, live-cell imaging, and drug screening for targets within the HASPIN-Aurora B pathway, relevant to cancer research. Case studies contain reports on ASCENT's exploration of the underlying mechanisms of cell states, including cell growth, cell differentiation, and even cell death.
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